
Lesson Goals
- To extract and purify plasmid DNA from a culture of E. coli
Key Terms
- Miniprep Spin Column
- Resuspension Buffer
- Cell Lysis Buffer
- Neutralization Buffer
- Ethanol Wash Solution
- Supernatant
- Precipitate
- Pellet
Protocol
SBone Protocols: DNA Minipreps
Optional Links and Further Reading
Protocol for Qiagen miniprep kit.
Protocol for Promega miniprep kit.
Course Curriculum
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Introduction
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Unit 1: Meet the Plasmids
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Unit 2: Transformations
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Unit 3: Measuring and Modeling Growth
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Unit 4: Comparing GMO Opinions
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