Course 2: Your First GMO

Lesson Goals

  • To extract and purify plasmid DNA from a culture of E. coli

 

Key Terms

  • Miniprep Spin Column
  • Resuspension Buffer
  • Cell Lysis Buffer
  • Neutralization Buffer
  • Ethanol Wash Solution
  • Supernatant
  • Precipitate
  • Pellet

 

 

Protocol

SBone Protocols: DNA Minipreps

Optional Links and Further Reading

Protocol for Qiagen miniprep kit.

Protocol for Promega miniprep kit.

 

 

 

 

Course Curriculum