Course 2: Your First GMO

Creating a GMO is as easy as cooking an egg. In this project we will produce simple transgenic organisms by transforming plasmids, tiny circles of DNA, into live E. coli bacteria. The resulting microbes are useful mainly as a DNA factory, growing and replicating more DNA for us to extract and manipulate in the lab. This same technique will allow us to introduce small genetic devices into bacteria quickly and cheaply.

Goals for this Course

  • To introduce plasmids as a tool for carrying DNA into cells.
  • To demonstrate lab protocols for moving DNA around bacteria.

Course Curriculum

  • Introduction

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  • Unit 1: Meet the Plasmids

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    • The Parts of a Plasmid
      30m
    • A Digital Plasmid in Sequence Editor Software
      30m
    • Quiz2.1
      Plasmid Quiz
      6 questions
  • Unit 2: Transformations

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    • How to Make Chemically Competent E. coli
      30m
    • How to Transform E. coli by Heat Shock
      30m
    • How to Harvest Plasmid DNA with Minipreps
      30m
    • Quiz3.1
      Miniprep Quiz
      5 questions
  • Unit 3: Measuring and Modeling Growth

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    • Measuring Bacterial Growth by Optical Density
      30m
    • Let’s Model Exponential Growth
      30m
    • Let’s Model Logistic Growth
      30m
    • Quiz4.1
      Growth Quiz
      7 questions
  • Unit 4: Comparing GMO Opinions

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    • A GMO Opinion: The AAAS
      30m
    • A GMO Opinion: Jason Kelly
      30m
    • Logistic Collector
      30m
    • Quiz5.1
      Logistic Collector Victory Code
      1 questions

Instructors

I am Jake. This is my profile.